PERIVISCI K AI FLUID OF ECHIXODERMS. 233 



The endoplasm of nearly all of the active leucocytes is granular 

 and opaque, the exceptions to this opacity are found in the leuco- 

 cytes of Ophiopholis acnleata (Fig. 9) and Cucnmaria japoi: 

 (Fig. 12 i. In these leucocytes the nucleus- with its content of 

 large chromatin granules is easily discernible. The endoplasm 

 tonally contain- a varying number of hyaline vacuoles which 

 -tain blue with the methylene blue-seawater solution and red with 

 the neutral red-seawat ition. 



The active leiiro, vie i- a phagocyte and is always found loaded 

 with <annine or India ink particles when those subMamv- are 

 introdured into the peri\ i-< eral cavity. In the Holothuroidea 

 the-e pha^' re "b-erved to deposit the india ink parti* 



in the skin (Srlmlt/. . \werinxew ('n) considers that the 



r of th- in M.ine of the Echinoidea is due to this pliago- 



cytic activity of "aiiio >" and that the color difference in 



\aiietir- df .s'. drobachi is dependent upon tlu- color of the 



fond, the pigments of which are taken up by the "am<eboi-\ 

 and i arried to tin- -kin. Since the leucocytes are the only type- 

 of < rlls in the peri\i-vral tluid which are pi tic, they 



\\ithoilt doubt the j>h :c "anio?lx)C\ activi 



are <!<-' iii'< <l b\ these and "ther authors. A further function of 

 the pli the removal of germ cell- remaining in the 



j;onads after the bulk of the ^ i metes have been >hed (Caullery 

 and Siedlii-ki. \\\ . ( 'erno\ odeanu and Henri (\>(>\ <b>erved 

 that bai teria inj( into the body cavity of sea un liin- W 



taken up 1>\ "ain-r: h long pseu<lo|)odia, '\-lls which 



are donbile-- K-ur.u\ti ^.nce mention ha- been made above 

 of the relation < ,f the lei; s to the transfer of f. M id i)roduct>, it 



i- pertinent to en|uiri- arther obserx at ion- ..f their |arti( i- 



palion in tlii- pha-e of \it.il at tivity. 



("uenot ('<)ib . one of the tirst to call attention to this relation, 

 a--nmed that the sub-lance- passed from the intestinal cells into 

 the inte-tinal lacun.e are taken up by the "amoeboc> ind 



stored up in them t<> be arried to other parts of the body. The 

 " amoebocytes " which are so com erned become metamorphosed 

 into "amoebocytea with spherules." Frenzel ('92) was of the 

 opinion that the " anwebocytes " pu>hed between the epithelial 

 cells of the intestine and into its lumen where they disintegrated 

 anil their remnants acted as a digestive ferment. Enriques ('02) 



