3O2 CHARLES T. BRUES AND RUDOLPH W. GLASER. 



by the single cells and another by the mycelia and asci, the latter 

 perhaps Alternaria tennis, a parasitic fungus that attacks various 

 Coccids of the genus Lccanhnn. 



Lindner (1895) found in an European scale insect (Aspidiotits 

 ncrii) a yeast-like organism which he regarded as related to 

 Saccliaroin\ccs aplculatus. By crushing one of the insects be- 

 tween a slide and cover-glass he observed large numbers of the 

 yeasts, both between the small masses of fat-body and actually 

 in the adipocytes. The organisms he described and figured as 

 usually very long, pointed at one end, or lanceolate, sometimes 

 joined in pairs by their acute tips, and frequently budding after 

 the manner of yeast cells. At that time Lindner was unable to 

 cultivate the organism although he evidently regarded it as a. 

 parasite, naming it Saccharomyces aplculatus, var. parasitus. He 

 found it forming a mass at the posterior pole of the egg and made 

 an ingenious explanation for its presence there, suggesting that 

 one of the pointed tips perforated the egg and gave off a bud 

 which then multiplied to form the mass or mycetome. 



A later paper by Lindner ( '07) which appeared in the Woch- 

 cnschrift filr Braucrcl we have not seen, but from published 

 reviews of this, it appears that it contains nothing bearing on the 

 physiology or systematic position of his yeast-like organism. 



Berlese ('06) studied in detail an organism which he found in 

 Ceroplastcs ruscl, and was successful in growing it on artificial 

 media. From Berlese's account, it appears that the fungus, 

 which he calls Oospora saccardlana is very similar to the one 

 described by us in the present paper. The yeast-like cells in the 

 Coccid vary in length from 4-12 //,, sometimes attaining a length 

 of 16-18/4 in early summer, agreeing in size and also in form 

 with those we have observed in Pulvinaria. In culture there is z 

 great similarity in the general morphology and development of 

 mycelia, and although Berlese gives few details concerning cul- 

 tural characteristics, at least one statement shows a striking dif- 

 ference between the two. He found that although the symbiont 

 from Ceroplastcs grows rapidly on gelatine media, that these arc 

 not liquified, while as will appear from our account, the Puh'l- 

 naria fungus exhibits a powerful liquefactive action on gelatine. 



