FUNGUS OCCURRING IN PULVINARIA IN NUMERABILIS. 3! I 



tion at room temperature (four weeks), the growth becomes very 

 flocculent and adheres to the surface of the glass where it is 

 finely dotted with very dark, pigmented spots. 



Moli-sctis Solution. After 48 hours rather good growth ; after 

 72 hours, no clouding or surface growth, but with very abundant, 

 slightly viscid sediment, no odor. 



Sugars. We have grown the organism in six sugars : lactose, 

 dextrose, mannite, saccharose, levulose and maltose. None of 

 these, however, furnish any differentiating characters ; in all 

 there is good growth without the formation of gas, but with 

 heavy viscid sedimentation, and after prolonged incubation, the 

 development of a distinct surface film. 



Milk. Growth is abundant, and after four to six days incuba- 

 tion the milk begins to clear at the top, sediment collecting in the 

 lower half of the liquid. Litmus milk becomes distinctly red at 

 the time of clearing. After about a week, the liquid becomes 

 whey, with a sediment at the bottom of the tube. 



Anaerobic Media. We have not been able to cultivate the or- 

 ganism under anaerobic conditions. 



PRODUCTION OF ENZYMES. 



Protease. Gelatine is rapidly liquefied. Milk cultures, tested 

 after 20 days, give a positive reaction with MgSO 4 , NaOH and 

 CuSO 4 , showing the presence of peptones. 



Lipase. After both 20 and 30 days' incubation, cultures in 

 either whole or skimmed milk give positive reactions. There is 

 a strong odor of butyric acid. Ethyl butyrate is also decomposed 

 with the formation of butyric acid. The 3O-day culture was 

 tested with pyrogallol and stannic chloride and gave a positive 

 reaction also. 



Diastase. Bouillon cultures after ten days' incubation were 

 treated with starch paste at incubator temperature for 48 hours ; 

 after this, Fehling's solution was reduced, demonstrating the 

 presence of sugar. 



MORPHOLOGY OF THE CULTIVATED FUNGUS. 



The cultures show during the first few days only yeast-like 

 budding cells like those seen in the early spring in the fat-body 



