78 JUSTIN M. ANDREWS. 



axostyle, after the preparation is stained, much better than 

 others. It is much more apparent in individuals fixed in osmic- 

 acid vapor, chromic acid, and strong Flemming's fluid, than in 

 Schaudinn's fluid, Carnoy's fluid, and Gilson's fluid. It shows 

 very well in specimens stained with Wright's stain. At its 

 anterior end, where it passes under the nucleus, it expands into a 

 capitulum at least twice its lower width, and then tapers rapidly 

 to a point where it is connected to the centroblepharoplast. 

 Passing from its enlarged portion, the axostyle almost invariably 

 takes a bend of at least 90 degrees. The particles of cytoplasm 

 surrounding the organelle at this point stain more deeply than the 

 rest of the proximal cytoplasm (Fig. I, A). The axostyle does 

 not seem to be a cylindrical rod. It is flat, and, at its posterior 

 end, it is sharply pointed. It has been observed in some prepa- 

 rations to be folded back on itself after it has pierced the body. 

 Frequently the trunk is not parallel for its entire length but 

 gradually enlarges from its most narrow portion directly posterior 

 to the capitulum to its maximum width near the posterior end 

 (Fig. i, A). Then it tapers at once to an acute point. In some 

 other cases, the width of the trunk is the same throughout its 

 length. 



The granules of the axostyle axostylar chromidia (Axo. chrom., 

 Fig. 2) stain intensely, and it is by their aid that the axostyle is 

 recognized more frequently than by its own outline. They are 

 sometimes arranged in a straight line (Fig. 3, A), occasionly they 

 are arranged in spiral rows, (Fig. I, A), and not infrequently one 

 finds them scattered throughout the organelle in an apparently 

 hit-or-miss fashion. 



i 



The literature concerning the parabasal body (Par. b., Fig. 2) is 

 so well reviewed in previous papers (Kofoid and Swezy, '15, 

 Cutler, '19) that it is sufficient to state in this paper that Janicki 

 ('n, '15), Alexieff ('13), Kuczynski ('14), Cutler ('19), and 

 Wenrich ('21) have all reported the presence, in various tricho- 

 monads, of darkly-staining, club-shaped organelles in addition to 

 the elongated, slender, chromatoidal rod that lies under the 

 undulating membrane. Kofoid and Swezy ('15) are inclined to 

 regard this as the first step in the origin of the new chromatic 

 basal rod in the prophase of fission. The figures of the developing 



