JUSTIN M. ANDREWS. 



In the meanwhile the centrosome has split, and the two portions 

 migrated apart, spinning out between them an intensely staining 

 bar, the paradesmose. By the elongation of the rhizoplasts con- 

 necting the centrosomes and blepharoplasts, the paradesmose 

 descends to the nuclear membrane. As it approaches the mem- 

 brane, the chromatin within begins to arrange itself in linear 

 fashion perpendicular to the paradesmose, and shortly after, the 

 paradesmose comes in contact with the membrane, and is greeted 

 by a projection of chromatin that is thrust up from the central 

 mass. At this time, the subparadesmosal fiber appears (Fig. 4, 

 D) upon which the chromosomes seem to arrange themselves 

 shortly after (Fig. 4, ). Probably the chromosomes divide at 

 this time while they are in this position, for the next figures we are 

 able to find show them being gradually drawn to the ends of the 

 paradesmose, without any indication of spindle formation. The 

 number of chromosomes is difficult to determine, but is somewhere 

 around ten. . The chromosomes form irregular clusters or rosettes 

 at the ends of the paradesmose, which always seems to maintain 

 itself as a fairly straight line (Fig. 5, C). The two masses of 

 chromosomes continue to migrate apart during anaphase (Fig. 5, 

 D] and the paradesmose persists. In telophase, the nuclei assume 

 their characteristic ellipsoidal shape, surrounded by hyaline zone 

 and nuclear membrane, and the paradesmose gradually fades 

 away. The two animals now separate by a plasmotomy, which 

 is materially aided by the activity of the locomotor apparati of 

 the two individuals. 



The division of the axostyle we have not been able to observe- 

 but from its capricious staining reactions during prophase, it 

 seems probable that it may be absorbed, and new ones grown out 

 from the blepharoplasts. By telophase, the two axostyles are 

 complete. 



Multiple Fission. 



Multiple fission seems to occur rarely in Trichomonas termop- 

 sidis. In the material worked on, fifteen or twenty cases were 

 observed, but they were all on one slide that is, from the in- 

 testinal contents of one termite. There were two types of 

 multiple fission, the one where the nuclei were in a polar position 

 (Fig. i, C), and the other where the nuclei were in a central 



