MORPHOLOGY IX TRICHOMONAS TERMOPSIDIS. 77 



attached directly to the chromatic basal rod. This observation 

 is strikingly demonstrated in those cases where the cytoplasm of 

 the animal has been destroyed, and the entire neuromotor system 

 with, or without the nucleus, remains intact. In the majority of 

 such cases, though not in every one, the undulating membrane 

 remains adjacent to the chromatic basal rod suggesting that 

 there may be some connection between them which is strong 

 enough to withstand the treatment that destroys the cytoplasm, 

 but which cannot, in a few cases, endure the chance violences of 

 the smearing operation. 



The axostyle (Axo., Fig. 2) in Trichomonas termopsidis is one of 

 its most conspicuous features when the animal is studied in the 

 living state. As the flagellate moves about, turning and twisting, 

 one of the first things to be noted is the projecting trunk of the 

 axostyle. Occasionly it sticks out of the body for a distance as 

 long as the length of the body, but more frequently it barely 

 protrudes through the cytoplasm, the length of the extruded 

 portion being varied both by changing the actual linear expanse 

 of the organelle which is accomplished by bending it and curving 

 it within the body but more often by changing the shape of the 

 body, so that more of the axostyle is included at one time than at 

 another. 



When the stained preparation is observed, the axostyle is not 

 such a prominent feature. It does not stain well at all. Fre- 

 quently it does not project beyond the body line, and in such 

 cases it is easily overlooked. Certain fixing fluids show the 



FIG. 3. All figures of Trichomonas termopsidis sp. nov., from material stained 

 with iron alum haemotoxylin, drawn with the aid of camera lucida. Flagella are 

 shown as they appeared in the preparation. 



A. Trophozoite in early prophase blepharoplast just divided, and flagella sepa- 

 lating into two bundles. Fixed in osmic acid vapor. X 2750. 



B. Prophase, showing new chromatic basal rod and undulating membrane 

 growing out from daughter blepharoplast. Fixed in Schaudinn's fluid. X 1200. 



C. Later prophase, showing parabasal body split almost entire length. Fixed in 

 i per cent, chromic acid. X 500. 



D. Late telophase, all structures duplicated, and animals ready to separate. 

 Note flattened, twisted appearance of parabasal bodies also the pellucid margin of 

 the undulating membrane. Fixed in I per cent, chromic acid. X 1000. 



E. Slightly earlier stage of telophase nuclei still connected by paradesmose. 

 Fixed in Flemming's strong solution. X 1300. 



F . Isolated neuromotor system, dry fixed, and stained with Wright's stain. 

 X 1150. 



