7O JUSTIN M. ANDREWS. 



ACKNOWLEDGMENTS. 



Many thanks are due Dr. R. W. Hegner for his valuable sug- 

 gestions and criticisms. To Dr. L. R. Cleveland I am especially 

 indebted for furnishing the material used, for suggestions as to 

 methods, and for the oversight which he so generously gaVe. 



METHODS. 



In order to obtain preparations which contained more than the 

 usual number of the flagellates per unit area, the termites were 

 starved for eight days, at which time the two larger protozoa, 

 Trichonympha campanula and Leidyopsis spliaerica, which had 

 been previously present in large numbers, had entirely disap- 

 peared (Cleveland '25). Then the termites were fed a pure 

 cellulose diet (Whatman Filter Paper No. 43) which resulted in a 

 much greater clarity of the intestinal fluid surrounding the 

 flagellates and relieved the animals of many food inclusions (wood 

 particles) that had previously obscured their internal morphology- 



Smears were made from the intestinal contents of termites 

 treated as above. They were fixed in various fluids, Schaudinn's 

 strong Flemming's, Gilson's, Bouin's, Zenker's, Carnoy's, osmic 

 acid vapor, and chromic acid. These were stained, for the most 

 part, with Heidenhain's iron alum hfemotoxylin ; a few were 

 stained with Mallory's Tri-Stain. Others were dry fixed and 

 stained with Wright's . stain. Sections of the intestines of 

 termites both of individuals that had been partially defaunated 

 as above, and of untreated hosts, were fixed in Schaudinn's fluid, 

 Flemming's fluid, chromic acid, and osmic acid vapor. They 

 were stained with iron haemotoxylin, and with Mallory's Tri- 

 stain. The live animals were also studied under vaselined 

 coverslips. 



MORPHOLOGY. 



Shape and Size of Body. 



Body shape in Trichomonas termopsidis is exceedingly variable' 

 due to the absence of a restraining pellicle, and to the extreme 

 lability of the cytoplasm. From a pyriform shape, it varies 

 through a regular and elongated oval, to a spheroidal contour. 

 The greatest width is usually near the middle of the animal, and 

 varies, in proportion to the length, from I to 4.5, to I to i. 



