96 CHARLES V. MOKKILL. 



In Anasa, a rounded cytoplasmic body was found, in two cases, 

 in or near the first polar spindle (Fig. 3, i; Fig. 4, c-d}. This is 

 perhaps comparable to the "eigenthiimliches Korperchen" which 

 Henking described in Pyrrhocoris. It may be a plasmosome, but 

 it is difficult to decide, since one frequently finds a number of 

 cytoplasmic bodies in the neighborhood of the first polar spindle 

 (PI. I., a) which cannot be distinguished from yolk granules and 

 which are inconstant in appearance and number. 



At the conclusion of the first polar division the spindle gradu- 

 ally fades away; there is no persistent cylindrical mass of spindle 

 fibers or "thelyid" as Henking ('90 and '92) found in Picris, a 

 lepidopteran, and Agelastica, a coleopteran. The chromosomes 

 left in the egg, as stated before, remain separate and there is no 

 telophase in the strict sense. After a short resting period, they 

 rotate about 45 and become disposed on a new spindle which 

 has formed out of the cytoplasm surrounding them. The axis 

 of the second polar spindle lies very obliquely to the surface of 

 the egg (Fig. 2, h and i; PI. I., b). As in the first division there 

 are no centrosomes or asters. In late anaphase a cell-plate is 

 formed by swellings of the spindle fibers. The second polar 

 body is constricted off in the same manner as the first and lies 

 alongside of it in the same depression. The first does not divide. 

 The two bodies finally become embedded in the surface cytoplasm 

 and can be distinguished as late as the third or fourth cleavage. 



At the close of the second polar division, the chromosomes left 

 in the egg become massed together and are converted into the 

 female pronucleus (PI. I., b). Those which have entered the 

 polar bodies may remain separate for some time but eventually 

 fuse into one or two deeply staining masses. 



C. Fertilization. 



The spermatozoa enter the egg through the micropyles which 

 form a conspicuous ring at the anterior end. Polyspermy is 

 undoubtedly normal, for accessory sperm nuclei were found in 

 the egg as late as the copulation stage shown in PI. II., h. As 

 many as three of these nuclei appeared in some cases. At the 

 time when the first polar spindle is in late anaphase, the -.perm 

 head enveloped in a mass of cytoplasm has moved some 



