N'AUCfLAK AH'AKATl'.- OF ANIMAL 



take in the hi- .tcuole of the latter. I think we are iu-u- 



r the present, to be sure, only as a working hy- 



hat t : -.v-rk of canal- found in so many animal 



(i-ll- i- the ph\ -i'.l-or and morphologic equivalent of the vacn- 



ol.ir '-11. 



\\'e ma- 'urn to the consii a of the question 



\\heiln-r tin- e.malii tilar -\-tem represents the true structure of 



the vacuolar mechanisn g plant cells or not. Thisquesti<>n 



f course, b ! -nl > l-\ observations of the living cells 



tin ni-i -1\< -. and the ii it ion of these is beset byextraordi- 



dittH ultie- in r cell due in particular to the 



impo ibiliu nidi in \\hirh to examine the cell-. 



\\hiih i-in.t it-i-l!' injurim!-. In theet't'ort to find a suitable fluid 



;hi- pin |" -< 1 tri( inin nitrate, of sodium 



rhlnndr. .im! ntrations, but found 



in all i hat t he -nrl.u .ipid changes of tin 



-true tun- <! tin !.i-m \\ hit h i 'ill to study t In 



uinaliK uninini' 5. 1 was linalU 



i.l>li;^( il tu re a mounting medium 



the ire-lil- of similar ti- (though even in this 



the t -ll- i-i tin rsoffree-hai underwent more 



-lip\\l\ tin , In these s so mounted the 



cell- -hn\\ed the iiuilti|)le \ aeuolar o >ndii i -n ili-cribed by Went. 

 In tin the contrary, in set >i the onion i 



tip, one emild see, \\ ith ditiu tilty to l>e sure, but still unmistakably 

 the canal system i entedii 1.2 and 3. As these cell- 



\\atehed. however, t !n ' anals are seen to break up slowly int" 

 munded \aeuli- thu- 1 about the ronditimi i:enerall\- 



1 in th -i . rll-. I n _trd, therefore, the canalicular - 

 ti in a- the in iition intra vitam of the vacuolar apparatus in 



ilu--e .-ell -of the n" -t lip. a ml believe that the multiply vacuol. 



lition i- !idar> origin due in most cases to injur 



the (ell. 



REFERENCES. 



i ; \vitli 



Holmgren, E. 

 '02 \ 



-.\ 



