LOCALIZATION OF NEW AXES IN CORYMORPHA. 



475 



conditions. Up to the present, however, only one experiment of 

 this sort under inhibiting conditions has been carried out, but its 

 results are conclusive. Of the twenty pieces each including the 

 naked and half the perisarcal region, which were used in this ex- 

 periment fourteen, seventy per cent, gave rise to basal structures 

 in the region of lateral injury and one, five per cent., to a hydranth, 



16 



18 



FIGS. 14-18. Cases of development under inhibiting conditions of basal 

 structures from lateral growing region determined by injury. Pieces in- 

 clude whole naked and half perisarcal region of 50 mm. animals. Figures 

 are about twice natural size. 



while five, twenty-five per cent., healed without outgrowth. Figs. 

 14-18 show characteristic cases from this series four days after 

 operation. In Fig. 14 the region of injury has developed merely 

 a few holdfast or stolon buds, while in Figs. 15 and 16 stolon buds 

 are present at the tip of a general basal outgrowth. In the case 

 of Fig. 17 the stolon buds in the region of injury are more numer- 

 ous and attain greater length than in any other case. In Fig. 18 

 the region of injury has given rise to two basal outgrowths. These 

 cases are sufficient to demonstrate that the lateral injury may 

 develop as a basal as well as an apical end. 



DISCUSSION. 



The experiments described above show that it is possible to 

 localize new polarities in Corymorpha stems even without pre- 

 viously obliterating or decreasing the original polarity by means 

 of inhibiting agents. It is important to note that these new polari- 



