SPERMATOGENESIS IN PARATETTIX. 267 



tributed around the periphery of the nucleus (Fig. 30). As 

 the tail becomes longer it becomes thinner and a filament extends 

 from the nodule of the head through the entire length of the 

 tail. Very little cytoplasm now surrounds the head (Fig. 31). 

 Finally the head becomes arrow shaped and stains very intensely. 

 The tail is long and filamentous and stains a little less intensely 

 than the head. The head and a portion of the tail are illustrated 

 by Fig. 32. The tail is more than four times as long as is shown 

 in the figure. 



McClung ('14) says: "It seems very evident that in the 

 spermatogonia of the Acrididae we are dealing with a chromo- 

 some complex of a very definite and precise organization which, 

 in the great majority, presents itself without essential variation 

 in number, size and form, fiber attachment, arrangement in the 

 metaphase and behavior during division of its elements. Steno- 

 bothrus and Pamphagns seem to be definite exceptions in some 

 of these respects. . . . And again he says: 



"With the exception of the Stenobothrus-like species, and 

 Pamphagns, the students of the Acrididae have reported a reduc- 

 tion of the 23 spermatogonial chromosomes to 12." 



All of the genera of the family Acrididae 1 discussed in McClung's 

 paper belong to the three subfamilies, Tryxalinae, (Edipodinae, 

 and Acridinee, and none belong to the subfamily Tettiginae. 

 It would seem that with the general agreement of the great 

 numbers of genera of this family which he and his students have 

 studied as well as those of other independent workers that he 

 would be justified in making the general statements concerning 

 the family. However, Paratettix leuconotus-leucotliorax of the 

 subfamily Tettiginae, show some exceptions. The spermato- 

 gonial number of chromosomes are thirteen instead of twenty- 

 three. The number of chromosomes in the primary spermato- 

 cyte is seven. This the writer has found to be true also for 

 both the parent forms of the hybrid as well as for others belonging 

 to the genus Paratettix. 



1 The writer is aware of the fact that there has been much shifting about of 

 names of the short-horned grasshoppers and that some taxonomists consider the 

 the grouse locusts of family value. If this should be the position which McClung 

 takes, then he would not consider Paratettix as belonging to the family Acrididae 

 and it would follow that the observations recorded in this paper would not be excep- 

 tions to his statements concerning Acrididae. 



