DISTRIBUTION OF SUBSTANCES IN SPERMATOZOON. 



247 



3. SCHUMACHER'S METHODS. 



A. 



One makes a smear with sperm as thick as possible, and 

 before the sperm is dried the slide is plunged into HC1 solution 

 I : 4. The slides remain in the acid 24 hours. After that time, 

 wash in distilled water and stain for 5 minutes in I per cent. 

 Victoria blue in distilled water. Again 5 minutes in distilled 

 water. Mount after usual treatment in cedar oil, or, in order to 

 have clearer pictures (but more unstable) one can mount directly 

 in water and seal with paraffin. 



TTl.p. 



FIG. 8. Spermatozoa of Arbacia prepared according to method A of Schumacher 



and stained in Victoria blue. 



The idea of this treatment is as follows: The various chemical 

 components of cells show a different resistance to the various 

 chemical agents. By combining the destructive agents and the 

 time of acting, it is possible to isolate by turn the chemical 

 components of the cell and detect them afterwards. 



Schumacher uses strong acids in order to destroy the proteins 

 of the cell. The remainder, after maceration, must be some 



