NOTE ON THE HEMOLYTIC ACTION OF SEBRIGHT 

 SERUM ON LEGHORN CORPUSCLES. 1 



MARY JUHN. 



In a series of experiments on grafts of Leghorn testes into 

 Sebright capons and Sebright testes into Leghorn capons carried 

 out by Mr. Roxas in this laboratory (i), the observations were 

 made that the Leghorn testis takes readily in the Sebright but 

 that the inverse is not the case. A much greater mortality was 

 also observed in the Leghorn hosts having Sebright grafts than 

 in the Sebrights having Leghorn grafts. In actual figures: 



Leghorn Testis Sebright Testis 



into Sebrights. into Leghorns. 



No. of birds 38 50 



Died from known causes 12, 31% 8, 6% 



Died, cause unknown 8, 21% 24, 48% 



Survived to end of exp 18, 47% 18, 36% 



Birds with succ. grafts (% of surviving 



birds) ii, 60% 3, 8% 



According to a verbal communication by Mr. Roxas, the 

 Leghorn capons with Sebright grafts that succumbed, showed 

 evidences of pronounced anaemia, this being evident in the lack 

 of color of the headfurnishings which became progressively 

 almost a dead white. 



In attempting to determine the causes for the greater mortality 

 among the Leghorn hosts as well as the reason for the much 

 smaller per cent, of successful grafts of Sebright testes into the 

 Leghorns, the following suggestions present themselves. Con- 

 sidering first the conditions in the Sebrights having Leghorn 

 testes grafts, the per cent, of takes is high ; the mortality which 

 may be attributed to the graft (cause of death unknown, 21 

 per cent.) is relatively not so pronounced. We may believe 

 then that the Leghorn tissues are readily incorporated in the 



1 From the Whitman Laboratory of Experimental Zoology of the University of 

 Chicago. The expenses of these investigations were supported in part by the 

 Committee for Research in Problems of Sex of the National Research Council; 

 grant administered by F. R. Lillie. 



474 



