476 MARY JUHN. 



host toxic substances resulting from the destruction of the 

 implant, and these toxic substances finally prove fatal to the 

 carrier of the graft. 



The point of interest is the reason for the different results 

 obtained in these cross-transplantations. The possibility of some 

 specific differences in the blood of the two breeds of fowl was 

 suggested by Prof. F. R. Lillie; the tests made to establish the 

 presence of such differences are described in the latter part of 

 the paper. 



My thanks are due to Professor Lillie both for suggesting the 

 problem reported here and for his continued helpful interest in 

 the work. 



Some experiments carried out by Sokoloff (5) serve to illustrate 

 that such conditions can be found in other vertebrates. Sokoloff 

 working on homotransplantations in rabbits found the presence 

 of three types of blood and states that grafts only take when 

 made into animals of identical blood constitution with the donor. 

 When grafts are made into animals where the serum of the host 

 agglutinates the corpuscles of the donor, the graft shows complete 

 necrosis. This destruction of the graft cells leads to the produc- 

 tion of specific antibodies in the host which have an unfavorable 

 action on the graft. Any graft will cause the formation of some 

 percentage of antibodies but these are counteracted by the graft 

 as soon as vascularization is established and they then disappear 

 from the blood stream. 



Furthermore according to Sokoloff, immunization by intra- 

 peritoneal injections of an emulsion of the organ to be grafted 

 causes the appearance of antibodies as well, and the presence of 

 these antibodies inhibits take and growth of the graft. 



In studying the conditions in the Leghorns and Sebrights, no 

 tests were made for the determination of circulating antibodies. 

 The sera of Leghorn and Sebright cocks and capons were tested 

 for their agglutinating and hemolytic action on the corpuscles of 

 all four kinds of birds. 



The experiments were repeated four times, the preparation of 

 the serum and the corpuscle suspension being identical in every 

 case. For the corpuscles blood was drawn from the ventricle 

 into a syringe moistened with a 1.5 per cent, sodium citrate 



