GYNANDROMORPHS IN HABROBRACON. 1 05 



as previously to male. Jabbed its protruded sting into cotton 

 plug again and again as is sometimes done by female. Reacted 

 toward a virgin female like a female toward a caterpillar, thrusting 

 out sting above her and below her and showing no flipping of 

 wings. 



December 4. Negative reaction toward male. Indifferent re- 

 action toward caterpillar. 



Tests of freak 144 show clearly changes in responses between 

 those characteristic of the male and those characteristic of the 

 female, the male type predominating. 



Freak 249. A female from orange defective L. stock 3 was 

 crossed with a male of orange L. stock 7. These stocks were 

 derived early in the history of the L. material and consequently 

 were not closely related. An Fi female after mating with her 

 orange defective brothers produced orange males 6 normal, 2 

 defective, orange females 12 normal, 8 defective, and an orange 

 gynandromorph with normal wings, freak 249, found in vial a 

 (February 8, 1924). 



The head of freak 249 was obviously male, having twenty- 

 one joints in each antenna. The abdomen was entirely female 

 both externally and internally. Ovaries, poison sac and glands 

 appeared normal. Seminal receptacle contained sperm, showing 

 that a mating had taken place. 



Tests for reactions extended for five days. When introduced 

 to females, the gynandromorph responded by all the typical 

 male reactions. A test with a male resulted in no reaction on 

 the part of either although the male mated immediately with a 

 female introduced shortly afterwards. Reactions toward cater- 

 pillars were altogether indifferent. 



Freak 246. After nine generations of inbreeding of descendents 

 of the impaternate female, freak 276, two branch lines were 

 bred for three generations separately. One gave rise to an 

 orange-eyed female heterozygous for wrinkled wings; the other 

 to an orange wrinkled male. A cross of these two resulted in 

 orange males, 7 flat and 5 wrinkled ; orange females, 4 flat and 6 

 wrinkled, and an orange wrinkled gynandromorph, freak 246, 

 found in vial a (January 4, 1924). 



Head was clearly male having twenty-three joints in left 



