A LIPO-GEL REACTION". 22J 



(6) Possibly the quantity of sodium chloride normally present 

 in the follicular fluid was lacking or reduced so that even after 

 the addition of o.i cc. sodium chloride solution the amount of 

 this salt present was still insufficient. 



(r) Possibly the proportions of the three substances used was 

 not perfectly correct as they were measured by drops usually. 



For all practical purposes the proportions of follicular fluid, 

 sperm and sodium chloride solution stated above are sufficiently 

 accurate and seldom fail to yield the reaction. 



3. After the conditions necessary for the production of the 

 coagulum had been determined, a new series of experiments 

 was undertaken to establish more definitely the nature of the 

 reaction. The results may be summarized briefly. 



(a) The reaction is not produced by other liquids of the 

 organism. I added follicular fluid or dry sperm to the following 

 body fluids: amniotic fluid, blood taken from the jugular vein, 

 defibrinated blood, smear of the mucous membrane of the uterus 

 and tube, urine, cerebrospinal fluid, aqueous humor of the eye, 

 vitreous body of the eye, peritoneal fluid, and pericardial fluid. 

 In all cases the result was negative. 



(b) The reaction is interspecific. The following combinations 

 were tried: 



Dry Follicular 



Sperm. Fluid. 



ram X cow 



bull X sheep 



goat X cow 



goat X sheep 



opossum X cow 



guinea-pig X cow 



ram X pig 



In all of these tests the result was positive. 



(c) Materials from different sources. Follicular fluid taken 

 from one follicle of the cow was tested with sperm taken from 

 the epididymes of ten different bulls. The result was positive 

 in all cases. Follicular fluid fiom ten different ovaries of the 

 cow was tried with dry sperm taken from the same epididymis 

 of a bull with positive results in seven cases only. Probably 



