DISTRIBUTION OF SUBSTANCES IN SPERMATOZOON. 24! 



more and more similar to those obtained by Victoria blue in 

 distilled water. 



Fuchsin (i per cent, distilled water solution): The affinity of 

 the fuchsin for the lipoids is less than that of Victoria blue. 



FIG. 4. Arbacia. Fresh spermatozoon in i per cent. Victoria blue in distilled 

 water Swelling of tail and twisting around head, a, middle piece; b, hydrophilic 

 substance; c, lipophilic substance. 



The staining is accordingly slower and one can follow this 

 process more gradually in the parts of the slide where the effect 

 of distilled water is moderated by a suitable mixing with sea- 

 water. 



The new fact obtained by this method is the possibility of 

 observing changes of shape and variations of size and form of the 

 middle piece. One can see in its region only granules varying 

 in number (2-6), or a ring varying in size from one spermatozoon 



