DISTRIBUTION OF SUBSTANCES IN SPERMATOZOON. 



245 



the best results in our experiments. It penetrates very slowly, 

 and shows excellent contrasts. 



By these stains one obtains more clearly the same results as 

 by Victoria blue in sea-water. With Janus green especially, 

 one obtains an entire series of pictures, which prove clearly 

 that there is a migration of the same substance which stains in 

 Victoria blue, to the connecting region between head and tail 

 and a large accumulation there of the migrating substance (Fig. 

 7 a-e). But here and there one can find spermatozoa in which 



FIG. 7. a-e, sperm heads of Arbacia showing stages of staining in Janus green 

 in sea-water; movements of Hpochromatic substance, f-h, Victoria blue in sea- 

 water, various conditions. The Hpochromatic substance is shown black. 



pare the action of Victoria blue and fuchsin in distilled water 

 (Figs. 2, 3, and 4) in which one sees the lipochromatic substance 

 also accumulated on various regions of the head. These facts 

 show the presence in the spermatozoon of an active cytoplasmic 

 layer, external to the nucleus, loaded with lipoids and taking 



the lipochromatic substance 1 migrates towards the apex. Com- 



1 I use this term lipochromatic substance for a succinct description, implying 

 only that the substance mentioned takes very strongly the stains reputed by the 

 work of Schumacher to be lipophiles: Victoria blue and fuchsin especially. 



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