HEMOLYTIC ACTION OF SERUM. 475 



Sebright organism, they soon become vascularized, and being 

 supplied with nutritive substances, persist and even show active 

 growth. There is no extended necrosis in the Leghorn grafts 

 beyond some presumably occurring when it is first implanted 

 and previous to vascularization. 



When Sebright testes are implanted into Leghorn tissues 

 however, there is a relatively high percentage of mortality 

 attributable to the graft (cause of death unknown, 48 per cent.) 

 and a very low percentage of takes, 8 per cent, of the surviving 

 birds and only 6 per cent, of all the birds operated upon. There 

 is no evidence of vascularization of the Sebright testis grafts, 

 on the contrary the appearance of the Leghorn capons after 

 Sebright implantations have been made lead one to believe in a 

 greater activity of the lymphocytes and a subsequent elimination 

 of the destroyed graft tissues into the blood stream of the host. 

 Blood counts before and after grafting as well as control of the 

 body temperature would presumably serve to determine the 

 accuracy of these assumptions. The resorption of the graft in a 

 large percentage of cases with subsequent mortality of the host 

 from one cause or another is however apparent. 



There is an extensive literature on the subject of the toxicity 

 of organ extracts when injected intraperitoneally, subcutaneously 

 or intravenously. The intravenous injections produce the most 

 rapid lethal effects, but subcutaneous injections of organ paste 

 into guinea pigs, were reported by Brieger and Ulenhuth (2) to 

 kill the animals within 24 hours after the injection. This effect 

 was correlated by Dold and Kodama (3) with the toxic action of 

 tissues in a state of destruction and with the causes of death 

 after burns. Pfeiffer (4) states that in acute cases of death from 

 burns, the cause of the mortality may be traced to toxic poisoning 

 induced by protein fission products. These products appear in 

 excessive quantities owing to the resorptive destruction of the 

 proteins which have been changed and killed through heat. 



It appears reasonable in view of the findings in comparable 

 fields reported above, to correlate the percentage of deaths in 

 the Leghorns having Sebright grafts with the non-success of 

 these grafts. The continuous resorption of the graft tissues, 

 may, and probably does, set free into the blood stream of the 



