1 66 IRVINE H. PAGE. 



Calculating Arbacia volume to that of Asterias = 2.527 cc. 

 N/io NaOH for 6,000,000 Arbacia eggs. 



Asterias Oil. An orange colored oil, non-drying, pungent odor 

 very much like vanillin, which has about the consistency of olive 

 oil. The 1 2 number (Wijs) was approximately 110-115 an d 

 saponification number 318.8. The saponification number and 

 the physical properties of the oil suggest that a much greater 

 proportion of high molecular weight fatty acids are present in 

 this oil than in that of Arbacia. 



The Asterias oil is present in great abundance while Arbacia 

 contains much less oil. This fact seems important. Distillation 

 of Asterias eggs with KOH gives very appreciable amounts of 

 H 2 S. Very little resulted from the distillation of Arbacia. 



PHOSPHATIDE FRACTIONS. 



1.539 grams of acetone insoluble material was obtained from 

 183 million Arbacia eggs. Much of this precipitate was insoluble 

 in boiling alcohol suggesting a high percentage of kephalin. The 

 presence of sphingomyelin was indicated by the presence of a 

 small amount of white precipitate on the addition of ether to the 

 alcohol solution. 



Acetone precipitates a large quantity of brownish white gummy 

 material from Asterias egg extract. It was largely soluble in hot 

 alcohol suggestive of a large proportion of lecithin. Further 

 examination of this fraction has been deferred. 



It is interesting to note that qualitatively large amounts of 

 soaps were found especially in the Asterias egg. 



i 



SUMMARY. 



1. The oil of the sea urchin egg (Arbacia punctulata) has an I 2 

 number of 146-148 and saponification value of approximately 

 606. The star fish (Asterias forbesii) egg oil I 2 number is about 

 110-115 and saponification value 318.8. 



2. Steam distillation of the eggs by the Dyer method suggests 

 along with the Kottstorfer number that the fatty acids of the 

 Arbacia egg are of a lower order than those of the Asterias egg. 



3. Qualitatively there appears to be more kephalin in the 

 Arbacia egg and more lecithin in the Asterias. The Asterias egg 



