iyo 



IRVINE H. PAGE. 



mixture was then filtered through ashless filter paper and the 

 filtrate used for analysis. The bone black should have removed 

 the majority of the echinochrome. 



RESULTS. 



Table I presents the average figure for the substance sought. 

 In every case at least three analyses were done. The results are 

 calculated on the basis of milligrams per one million eggs. 



TABLE I. 



ELECTROLYTE CONTENT OF ONE MILLION Arbacia EGGS. 



The weight per million eggs of the material dried to constant 

 weight at 100 degrees C. was approximately 0.124 grams, and the 

 ash was 8.5-10 per cent, of the dry weight of eggs. The Arbacia 

 egg averages 74.1 /JL in diameter (Glaser (6)). 



SiO 2 was not quantitatively determined but qualitatively 

 rather large amounts were found present. 



The distribution of the phosphorus between lipoid and inor- 

 ganic is discussed in another paper (fege (7)). 



The analysis of Asterias eggs was confined to the analysis of 

 the trichloroacetic acid filtrates described above. Due to the 

 greater size of the Asterias egg (103.6 micro for unfertilized eggs 

 (Glaser (6))) only 250,000 egg samples were analyzed. The 

 volume relation (4.1893) of the Arbacia and Asterias egg is 2.1- 

 5.2 or one million 404,000 eggs, hence although for convenience 



