KARYOKINETIC FIGURES OF CENTRIFUGED EGGS. 115 



hyaline band. The granules of the ectoplasmic layer are ap- 

 parently unaffected by the centrifugal force. The karyokinetic 

 figure has been moved little, if at all, as is proved by the fact 

 that there is an indentation opposite the outer pole, and by the 

 relation of the ectoplasmic layer which exhibits the character- 

 istic defect around the outer pole of the spindle, which I described 

 in my first paper. 



That portion of the karyokinetic figure lying within the baso- 

 phile cap partakes of its character, and is as sharply differenti- 

 ated from the remainder of the figure as the basophile cap itself 

 is differentiated from the protoplasm of the hyaline band. As to 

 the fiature of these differences: (i) the stain is much darker 

 within the basophile cap ; (2) the rays of the aster are more 



FIG. 9. Transverse section of parti-composition spindle. From section of an egg 

 of Chirtopterus centrifuged 1,150 revolutions in 31 seconds at a radius of 13 cm. 

 Half of the section of the spindle lies in the basophile cap. See text for further 

 description. 



numerous than in the control (Fig. i) and also more granular; 

 they are in fact obviously made up of linear arrangements of 

 basophile granules ; (3) the spindle itself is more darkly stained 

 within the basophile cap than outside of it ; this pertains to the 

 fibers themselves, but I would not venture to say that the fibers 

 are more numerous. 



Where a fiber either of the spindle or of the aster crosses the 

 boundary between the basophile cap and the hyaline zone its 

 character changes. Rays of the aster never extend into a compact 

 mass of acidophile granules, hence the present figure is less 

 developed on the right side than on the left. 



Fig. 5 represents a spindle placed tangentially to the basophile 

 cap. The contour of the spindle is perfectly regular, but its 



