314 



A. RICHARDS. 



were first described by Sommer in 1874, and named by him 

 " Nebendotter." This expression, which has the claim of priority, 

 seems unobjectionable except on the score of bringing a foreign 

 word into English ; no suitable translation has been suggested, 

 however. On the other hand, the body is not a true " neben- 

 kern," and to call it by that term is a misuse of the word. 



Fig. i is an early oogonium from T. scrrata. Here the 

 " Nebendotter" appears as an egg shaped body of even consist- 

 ency stained darker than the nucleus although lighter than the 



', 



I 2 



FIG. I. Oogonium, stained with iron hsematoxylin, showing " Nebendotter," 

 and nucleus with chromatin reticulum. 



FIG. 2. Oogonium showing same structures as Fig. I, but from a much later 

 generation. 



surrounding cytoplasm. Its reactions to various stains deserve 

 metion. With iron haematoxylin it stains readily, appearing as 

 a dark homogeneous mass even after a great deal of extraction 

 of the stain. The nucleus and cytoplasm may be entirely decolor- 

 ized and the "Nebendotter" still show as a dark body, a fact 

 which led to confusion during the early part of my study, as 

 the nucleus was overlooked and the " nebendotter " taken for it. 

 The true state of affairs was not revealed until I had used another 

 method of staining when the appearances which with iron haema- 

 toxylin had misled me were explained and the structure of the 

 cells became clear. The new reagents were Kernschwarz counter- 

 stained with Litchtgriin. Kernschwarz is a weak stain affecting 

 only the nucleus. In my preparations I have seen no trace of it 

 in the cytoplasm or in the " Nebendotter." Lichtgrun stains both 

 nuclear plasm and cytoplasmic structures. The result, then, with 

 this method of staining is as follows : chromatin and nuclear 





