MICROSPORIDIA PARASITIC IN MOSQUITO]-.-. IIJ 



immersion objective, but the emergence did not advance any 

 further. I cannot determine whether or not the form changed 

 during this period, since the spore underwent Brown's movements 

 which were further exaggerated by the turning of the finer 

 adjustment of the microscope. Some spores extruded while 

 under observation the filament from the attenuated extremity. 

 At the moment of the extrusion of the filament, the spore showed 

 a vi'o iroii^ vibration which seemed to have been caused by the 

 Midden unwinding and extrusion of the coiled filament. 



In tin l.ii\,r which were examined 40 hours after feeding on 

 the infected material, the mid-gut contained more empty -pores 

 than tho-e \\ith normal unchanged appearances. Some -[tores 

 \\ere -ecu \\ith extruded filaments (Fig. i,</). A number of 

 -mall amn-boid bodies were found in the vicinity of empty 

 -p"; i, e). This amoeboid body when first noticed wa- 



ne. ir three empty spores. It contained a small dark -put near 

 the center. While under observation, I saw it undent >ii . 

 -lu^i-h change of form. In the mid-gut of a larva which was 

 i j.\ h mis after feeding on the infected material, unimideated 

 1 .M<|ie- \\t n seen near empty spores (Fig. I,/). 



Since I was not able to completely follow the emergence of the 

 -pon>pla-m. I cannot state positively that these unimiele 

 l><><lie- \\ere emerged sporoplasms. The fact that they appeared 

 in a larger number as the empty spores increa-ed and that they 

 mbled losely in size and appearance t<> the intra-poral 

 >pnn.pla-m- and to the youngest stages found in the pcrimtc-tinal 

 adipo-c ti--ue of the host larvae, however, lead- me to consider 

 them as tin- sporoplasms which left the spores tinder the influence 

 of tin- di:^>tive fluid of the host. These anm-bula- are inn-tly 

 found in the posterior portion of the mid-.um where a number of 

 them \\ere l"und between the peritrophic membrane and the gut 

 epithelium (I'i;^. I, .?). How these airurbula- reachec) the adipose 

 oulil not be determined, although search wa- made 

 repeatedly. 



The >i>unv;c-t -chi/onts found in the adipose ti->u -urnainding 

 the mid-gut and the adjacent tracheae are shown in Fig. i, //, /. 

 They were observed in the larva? as early as 24 hours after 

 feeding on the infected material. Round in form, it shows 

 reticulated cytopla-in and a large and compact nucleus the 



