82 JOSEPH HALL BODINE. 



present experiments and positive reactions obtained only in the 

 alimentary canal. As a matter of fact no tissue outside of the 

 alimentary canal was found to give a positive reaction. The 

 reaction in the alimentary canal seems to be confined to the 

 caeca and stomach. It thus seems that for grasshoppers' blood 

 other data are quite desirable before definite respirators' functions, 

 similar to those for mammals, can be definitely attributed to it. 



SUMMARY. 



The blood of seventeen different species of grasshoppers 

 tested by means of appropriate indicators shows an average pH 

 value of 6.8. The average pH values for different parts of the 

 alimentary canal are: fluid emitted from mouth 5.5; crop 5.7; 

 caeca 6.2; stomach 6.4-7.4; intestine and rectum 6.4-7.2. 

 Oxidation tests for haemoglobin (guiac, P-diamino-benzene, 

 a-naphthol) have been obtained with the alimentary canal and 

 with no tissues outside of the alimentary canal. 



LITERATURE CITED. 



1. Crozier. 



'24 Jour. Gen. Physiol., Vol. VI., p. 289. 



2. Bishop. 



'23 Jour. Bio. Chcm., Vol. LVIII., p. 543. 



3. Jameson and Atkins. 



'21 Biochemical Jour., Vol. XV., p. 209. 



4. Felton. 



"21 Jour. Biol. Chem., Vol. LVL, p. 299. 



5. Clark. 



'22 The Determination of Hydrogen Ions, Second Ed., Baltimore. 



6. Muttkowski. 



'21 Annals Ent. Soc. America, Vol. XIV., p. 150. 



