MICROSPORIDIA PARASITIC IX MOSQUITOES. 115 



was the matter which occupied a great deal of my time. It was 

 found that this distinction could only be done with great difficulty 

 in the preparations stained with Heidenhain's iron haematoxylin, 

 while it was comparatively easily made out in the sections 

 -t, lined with Giemsa's mixture. With Giemsa, the nucleus of a 

 sporoplasm whether within the spore membrane or without, took 

 a peculiarly bright crimson color, which led me to distinguish it 

 I .- >--ibly as such from those of the other organisms present in the 

 lumen of the hist gut. 



The .-< lieme of examination of both the experiment and the 

 ( i mt nil l.ii \ ae was as follows: 2 



Timi- alti-r 



1 Observations in 



I ML Smears, 



.il. 



The alimentary canal of two larvae was Two huv.r \\iir lixfl 



extracted from the body and studied in toto. 

 in fresh conditions; later fixed and 

 stained. 



-4 In mis Two larvae observed in a way similar to Two larvae were fixed 



the above. in toto. 



..... larvae observed in a way similar to 



the above. 

 .... . . .The entire body of two larvae was studied Two larvae were fixed 



in fresh smears; later fixed and stained. in toto. 



.j . l.iys. . . Four larvae were fixed 



in toto. 



In the fresh preparations of the alimentary canal of the 

 lar\ .1 \\ hirh were examined six hours after feeding on the infected 

 material, a large number of unchanged spores with normal 

 appearances were observed. Spores with a comparatively large 

 lear area at the rounded extremity were noted in numbers 

 i, a); spores with a small mass projecting from the at- 

 tenuated end were also noticed (Fig. i, a). \Yhen stained, the 

 -pore- showed a relatively large nucleus (Fig. i, b). 



In the larva? which were examined 24 hours after feeding on 

 the infected material, the mid-gut contained a large number of 

 iinrhaii^ed spores and empty spores; some of the latter showed 

 extruded li laments. A number of spores exhibited an appearance 

 shoun in Fig. I, c in which apparently the emergence of the 

 content^ was taking place. This particular spore sketched here 

 was kept under observation for thirty minutes with an oil 



1 Due to unexpected early departure from the place, the experiment was unfortu- 

 nately discontinued on July 14. 



