356 HARVEY M. >MITH. 



that eating was impossible and the animal was in an extreme 

 state of starvation. The other specimens showed signs of having 

 recently eaten, and were well fed. They were kept at a tempera- 

 ture of about 12 C. when not being used for carbon dioxide 

 output tests. Attention is called to specimen No. 4, which 

 shows an unusually high carbon dioxide output. It is not in- 

 cluded in the general average for the species in Table VI II. 

 The detailed record for this animal follows: 2/10 0.0653, 2/29 

 0.0639, 3/ 2 0.1208, 4 18 0.1037, 4/ 20 o.i no (dates of 

 determinations and carbon dioxide output figures). No explana- 

 tion is found for this strange behavior; starting with approxi- 

 mately a normal carbon dioxide output value, jumping to twice 

 the normal, and remaining there. That the phenomenon is not 

 due to an acute infection is indicated by the fact that the animal 

 appeared normal on autopsy a month after the final test. 



Rana clamitans (Latreille). Two specimens were collected 

 near Madison and kept at room temperature until used three 

 months later. They had no food during this time. Blood 

 smears were made from No. I immediately after it was collected. 

 The low value for carbon dioxide production with the small 

 animal is probably due to the extreme emaciation of this speci- 

 men. Neither cell size nor activity results differ essentially 

 from those of the following species. 



Rana pipiens (Shreber). The specimens used were from 

 several shipments from supply houses. Some of these animals 

 were starved at room temperature for a long time, and the 

 effects of lack of food on metabolism were noted. No appreciable 

 decrease in metabolism resulted until the animal had reached 

 an extreme state of starvation. No. 2 lost 47 per cent, of his 

 body weight before any marked drop in carbon dioxide output 

 per gram of body weight was obtained. Several specimens died 

 of disease. The results of the study of metabolism during the 

 course of the disease will be described later. 



Rana palustris (Le Conte). The specimens used were collected 

 near Madison in the late fall. They were kept at room tempera- 

 ture and used for carbon dioxide output tests over a period ot 

 two months. At the end of this time they were all very weak 

 from starvation. Nos. I, 2, and 3 were killed while still in good 

 condition. No. 5 and Nos. 6-17 were tested when near death 



