204 



X. H. COWDRY. 



TABLE II. 



No. 



1570.. 

 I57L. 

 1572. 



1573- 

 1617. 



1627. 

 1626. 

 1566. 



1567. 

 1568. 



1569- 

 1625. 



1624. 

 1565. 

 1579- 



I579B 



Fixative. 



Radicle of Pea. 



Pancreatic Acini. 



Zenker o % acetic I day 



2-5% 



10% 



20% 



" 33% 

 50% 

 Regaud IV B, o% acetic 



5 days, mordanted 



in 3 % bichromate 



days 

 Regaud 2.5%acetic sday 



5% 

 10% 



Equal parts acetic and 

 water 



Pure acetic 



Benda no acetic 8 days 



Benda, 0.8% acetic, 

 mordanted in forma- 

 lin 20 c.c.; 3% bi- 

 chromate 80 c.c. 6 

 days 



Same but mordanted in 

 pyroligneous i day 

 and 2 % bichromate 2 

 days 



+better preserved in 

 older cells, apt to be 

 vesicular, fragmented 



+better preserved in 

 older cells, apt to be 

 vesicular, fragmented 



+almost all destroyed 

 in younger cells, still 

 fairly numerous in 

 older ones 



+more fragmented and 

 vesicular 



-ffairly well shown in 

 all cells 



+but very rare 



+normal 



+rather vesicular 

 + normal few 



+ normal few 



+normal 



+normal in peripheral 

 cells, absent in center 

 of tissue 



-(-fewer, only in peri- 

 pheral cells slightly 

 fragmented 



+same 



-fslightly fragmented, 



present in all cells 

 +but very rare 



-(-normal 



-(-normal 

 -(-normal 



-(-normal 



bichromate, and possibly also the sublimate, modify the action 

 of the acetic acid. In the deeper layers of the tissue', on the 

 other hand, we have to do with a successive action of the said 

 ingredients, determined by their relative rates of penetration. 

 The acetic acid penetrates first and acts upon the mitochondria 

 before the bichromate and the sublimate have come upon the 



scene. 



Mitochondria are surprisingly resistant to higher concentra- 

 tions. They are well preserved by a mixture of Zenker's fluid 

 and 20 per cent, acetic acid and their relations are shown in 

 Figs. 5 and 6. With 33 per cent, acetic acid only a few scattered 

 mitochondria remain, while they are completely destroyed by a 

 mixture containing 50 per cent, of acetic acid. 



