26 4 



CARL R. MOORE. 



Experiment : 



4:30 P.M. Eggs collected and washed. Aug. 26, 1916. 

 5:00 P.M. Divided into two lots A and B. 



A, placed in (50 c.c. sea-water + 8 c.c. 2\ m. NaCl). 



B, placed in (50 c.c. sea-water + 16 c.c. 2\ m. NaCl). 



Eggs were returned to sea-water from the two solutions by means of a pipette 

 and divided into two lots one of which remained without further treatment (con- 

 trol): to the other was added immediately a fresh sperm suspension. 



TABLE I. 



Lot A. 



Lot B. 



the number of cleavages or the number of swimming larvae. 

 But since an exposure of this intensity is practically ineffective 

 in producing initiation of development, is it not to be supposed 

 that the eggs can yet be fertilized by sperm? They are, so far 

 as we may judge, entirely normal or essentially so. However, 

 as the length of exposure to the hypertonic solution is prolonged 

 the capacity for fertilization drops off until after an exposure 

 of two hours' duration scarcely any larva? appear as a result 



