424 EDWIN G. CONKLIN. 



EXPLANATION OF PLATE IV. 



FIGS. 19-48 (with exception of Fig. 30) represent eggs which had been subjected 

 to hypertonic solutions. 



FIG. 19. No. 822. 2 per cent. NaCl, 16 hrs., normal sea-water 8 hrs. 

 Numerous karyomeres were formed after maturation and before the first cleavage, 

 presumably during the first cleavage mitosis. 



FIG. 20. Same slide as preceding. Karyomeres formed at the close of the 

 first cleavage by chromosomes failing to unite. Centrospheres (s) unusually large. 



FIG. 21. No. 837. f per cent. KC1 9 hrs.; normal sea-water 35 hrs. Karyo- 

 meres irregular in size, shape and distribution. 



FIG. 22. Same slide as preceding, with similar karyomeres. 



FIG. 23. No. 822. 2 per cent. NaCl 16 hrs.; normal sea-water 8 hrs. Second 

 cleavage abnormal, with karyomeres in two of the cells. 



FIG. 24. No. 814. 2 per cent. NaCl i hr. ; normal sea- water 17 hrs. Third 

 and fourth cleavages abnormal, with karyomeres in most of the cells. 



