432 EDWIN G. CONKLIN. 



EXPLANATION OF PLATE VIII. 



FIGS. 43-48 are different eggs from the same experiment, viz., No. 810. 3 per 

 cent. NaCl 15! hrs. All the eggs were in the anaphase or telophase of the second 

 cleavage at the time they were placed in the salt solution, and in all cases the 

 chromosomes have remained in the form of a dense chromatic plate or mass which 

 has not become vesicular. In Fig. 45, the spindle remnants between the chromo- 

 somal plates has become vesicular; in Figs. 46 and 47, the spindle area has become 

 an elongated achromatic vesicle, within which lies the dense chromatic mass; 

 in Figs. 43, 44, 48 the achromatic vesicle has become more nearly spherical in 

 outline, while the chromatic mass is not quite so dense as in earlier stages. These 

 figures show that when the chromosomes are prevented from absorbing achromatin 

 and becoming vesicular nuclei, the achromatin of the spindle region may become 

 a vesicle by the formation of a delicate achromatic membrane around itself. 



