234 B - C. H. HARVEY AND R. R. BENSLEY. 



that acid is present on the surface while it is absent from parietal 

 cells and from the gland lumen. 



Effects of Injury on Amount of Prussian Blue Precipitate 

 Second, is the amount of Prussian blue precipitate increased 

 by mechanical injury of the mucous membrane? A small kitten 

 was given by subcutaneous injection small doses of a mixture 

 of solutions of potassium ferrocyanide and iron and ammonium 

 citrate in molecular proportions. These injections were given 

 three times a day for three days. On the third day the abdomen 

 was opened, a small piece of mucous membrane was removed 

 from the fundus region of the stomach, the wound in the mucous 

 membrane was sutured with silk sutures and afterward the 

 other coats of the stomach were united and the abdomen w r as 

 closed. The operation was done with aseptic precautions. Five 

 hours later the kitten was killed, pieces of tissue were fixed in 

 alcohol and in neutral formalin (freshly distilled over potassium 

 hydrate). In the immediate vicinity of the suture paraffine 

 sections showed that the Prussion blue reaction occurred in great 

 abundance in the blood vessels, in the lymphatic spaces and in 

 the parietal cells. These preparations showed blue in a very 

 large proportion of the parietal cells and even in those at the 

 bottom of the tubules. It was especially abundant in the 

 parietal cells of the necks of the glands. Many parietal cells 

 were dead and thrown off into the gland lumen; in every instance 

 these dead cells were filled with Prussian blue. In those cells 

 which were still in their normal position many showed the 

 Prussian blue in the canaliculi disposed in a manner very similar 

 to that which Miss Fitzgerald has shown in her Plate VII., Figs. 

 6 and 8. As one proceeded in the study of these sections pro- 

 gressively farther from the site of injury the amount of Prussian 

 blue and the number of parietal cells showing it progressively 

 decreased, and in some sections of mucous membrane taken 

 from parts of the stomach remote from the site of operation and 

 apparently in a healthy condition, the parietal cells did not con- 

 tain any blue at all. 



In another cat a similar operation was performed on the gastric 

 mucous membrane and during four successive days following 

 solutions of potassium ferrocyanide and of iron and ammonium 



