INTRODUCTION. 25 



amount in the syringe is forced out in ten minutes. 

 While the injection is being made the limb should be 

 gently massaged by pinching and rubbing from the 

 foot toward the body. This facilitates the flow of the 



j 



liquid in the lymph-vessels. In this manner the lymph- 

 atics of each limb are filled. The author has been 

 able to fill the left thoracic duct by continuing the in- 

 jection for about twenty minutes in the palm of the 

 left paw. The lymphatics of the head and neck may 

 be injected in a similar manner by inserting the cannula 

 beneath the skin on the tip of the ear, the tip of the 

 tongue, the tip of the nose, and the lips. 



In order to inject the lacteals the abdominal cavity 

 must be opened, and the cannula inserted very obliquely 

 into the w r all of the small intestine so that its point is 

 between the mucous and muscular coats. The half- 

 filled syringe being attached, very gentle pressure should 

 then be exerted on the plunger, until the blue solution 

 appears in the lacteals. To inject all the lacteals it is 

 necessary to insert the cannula into the wall of the 

 intestine at a dozen different places. To fill the thoracic 

 duct injection may be made into the large lymphatic 

 gland lying at the point where the converging blood- 

 vessels of the mesentery meet. The best solution for 

 injecting the thoracic duct is made by dissolving 7 

 gm. of gelatin in a warm Berlin blue solution (4 gm. 

 of Berlin blue to 100 c.c. water). This should be filtered 

 through a single layer of absorbent cotton and then 

 injected while still quite warm. In all cases injections 

 to fill the lymphatics must be warm and must be pushed 

 in very slowly. These lymphatic injections are best 

 preserved by injecting the trachea and intestine with 

 95% alcohol and immersing the cat in a jar of 70% 

 alcohol. 



