I/O PREPARATION OF THE SUBSTRATES 



serum of carcinomatous subjects, or of individuals 

 with salpingitis, tuberculosis, and the like. Carci- 

 noma is correctly prepared if it is not attacked by the 

 serum of pregnant individuals. 



Above all, the organ should be tested by means of 

 cases which contain ferments acting against the com- 

 ponents of the red blood corpuscles. Cases of blood 

 effusion are excellent testing agents for the absence 

 of blood in the prepared organ. Or disharmonious 

 blood in this case human blood is injected into 

 an animal, and its serum is tested against 

 coagulated red blood corpuscles and the organ to be 

 employed. 



In conducting these experiments we must be able, 

 with absolute certainty, to prevent the decomposition 

 of all proteins other than those belonging to the 

 actual organ itself. It is clear that serum, which 

 contains a defensive ferment against the components 

 of the form-elements of the blood, will decompose every 

 organ containing blood that is, it will split up, not 

 the proteins of the organ, but the components of the 

 blood within the organ. The importance of a clear 

 recognition of this circumstance may be gathered 

 from the fact that serum of normal horses and cattle 

 decomposed red blood corpuscles in about 40 per cent, 

 of cases. Further, it was found that serum taken 

 from animals that exhibited hagmatoma produced 

 decomposition with every kind of organ containing 



