1/6 PERFORMANCE OF THE EXPERIMENT 



It is sufficient to use 15 to 20 c.c. of blood. For 

 sending away, only serum should be used which has 

 been centrifuged completely. The latter must in any 

 case be centrifuged again. The serum should not 

 be more than twelve hours old, even though it 

 has been collected and preserved in a really sterile 

 way. The taking of the blood, its collection, and its 

 manipulation must be done aseptically. 



PERFORMANCE OF THE EXPERIMENT. 



In carrying out a dialysation test the following 

 fundamental rules must be obeyed, of which not one 

 is unimportant : 



(1) Extreme cleanliness is the first condition for 

 ensuring success in the experiment. This applies 

 to the surroundings, and to all the utensils employed. 

 Pipettes, test-tubes, Erlenmeyer flasks, &c., must 

 be thoroughly cleansed and absolutely dry. 



(2) The water used must be thoroughly sterilized 

 distilled water. So-called distilled water often proves 

 to contain numerous germs of every description. If 

 one uses water like this as the outer fluid in dialysis, 

 then the way is laid open for all kinds of mistakes. 



(3) The work is performed, as far as possible, 

 aseptically and antiseptically. 



(4) In the room in which the experiments are in 

 progress neither bacteriological nor chemical work 

 should be allowed. Above all, an incubator must be 



