SOURCES OF ERROR (IX THE PROCESS) 



Three series of experiments were conducted with 

 the same sera, and with equal quantities of these. 

 In the first experiment the organ was -- ; o, i.e., it was 

 absolutely free from substances which could be boiled 

 out and filtered, and which, under the strictest con- 

 ditions, would produce a coloration with ninhydrin. 

 In every case we had to add to the quantity of 

 substances emanating from serum alone, and passed 

 into the dialysate, o gr. of these compounds. Then, 

 in the experiment serum organ, the ninhydrin 

 reaction obviously remains negative. 



For the second experiment an organ was taken, 

 which passed over to the boiled water just a trace 

 of reacting substances. \Ve will assume that it 

 contained o'lo gr. 19 of these compounds. This quan- 

 tity is added to that which the serum gives off, and 

 we have the positive reaction of Cases 6 and 8. The 

 limital value, i, has been exceeded. Thus, by means 

 of a simple addition, a positive reaction has been 

 obtained and, in consequence, two errors in dia- 

 gnosis. The third column shows us how the ninhydrin 

 reaction results, when we use an organ prepared in a 

 still more imperfect manner. 



Exactly the same position is reached, if the dialysate 



We take this here merely by way of an example. 

 Obviously, in actual tests, the same quantity, i.e., o"io gr. , 

 would never be transferred to the dialysate, if the organ can 

 only give off that amount; some lesser quantity would pass 

 over. 



