214 APPENDIX. 



ii. Mount a few cells in Schulze's solution and notice 

 that the cell-wall is stained blue (cellulose). 



II. Spirogyra. 



iii. Carefully examine some Spirogyra to find conju- 

 gating filaments, and make sketches of the various stages 

 of conjugation, viz. : 



a. the formation of protuberances on cells of con- 

 tiguous filaments ; 



b. their approach and impact, and the concentration 

 of the cell contents ; 



c. the flattening of the wall now common to the two 

 protuberances, and their bulging at the point of contact. 

 The cell protoplasm is now passing into the protuber- 

 ances. 



d. the passage of the protoplasm of one cell through a 

 hole produced by the solution of a part of the dividing 

 wall of the protuberances, and the consequent formation of 

 the zygospore. 



e. the thickening of the wall of the zygospore. This 

 however may not be shewn in material examined early in 

 the year, i.e. before June. 



III. Mucor. 



iv. Mount in water a small piece of Mucor grown on 

 gelatine. Sketch a portion of a young hypha, shewing 

 the cell-wall, and the protoplasm containing numerous 

 vacuoles ; these are smaller towards the tip, where indeed 

 they may be absent. 



v. Make a sketch of young sporangia, some contain- 



