4 PROTOZOA. 



Doflein: Lehrbuch der Protozoenkunde. 3d Auf., 1911. 



Edmondson: Protozoa of Iowa. Davenport Acad. Sci., 1906. 



Hartmann: Praktikum der Protozoologie, 1910. 



Jennings : Behavior of the Lower Organisms, 1906. 



: Old Age, Death, and Conjugation in the Light of Work on the Lower 



Organisms. (Harvey Lectures), Pop. Sci. Mo., 1912. 

 Kent: Manual of the Infusoria, 1881. 

 Lankester: Treatise on Zoology. 1. Protozoa. 

 Leidy: Fresh Water Rhizopods of North America, 1879. 

 Minchin: Protozoa, 1912. 

 Prowazek: Einfiihrung in die Physiologie der Einzellgen (Protozoen), 1910. 



: Taschenbuch der Mikroskopischen der Protistenuntersuchen, 1907. 



Stokes: Contribution Toward a History of the Fresh Water Infusoria of the 



United States. Jour. Trenton Nat. Hist. Soc., 1, 1888. 

 Whipple: Microscopy of Drinking Water, 2d ed., 1910. 

 Woodruff: Observations on the Origin and Sequence of the Protozoan 



Fauna of Hay Infusions. Jour. Exp. Zool., 12, 1912. 



RHIZOPODA. 



AMOEBA PROTEUS. 



Amoebae are usually easily discernible under the low power of 

 the microscope as irregular, semi-transparent, granular bodies. 

 Find a specimen in the material provided, which is known to con- 

 tain amoebae, and determine the following points: 



1. With the high power observe the peculiar method of loco- 

 motion, the constant but slow change in the shape of the body 

 by means of projections, pseudopodia, or "false feet." 



Make sketches at intervals of one or two minutes to show the 

 changes in the form of the body. 



2. Observe the peripheral zone of hyaline protoplasm, the 

 ectoplasm, and compare this with the inner protoplasm, the endo- 

 plasm. Observe in detail the formation of a pseudopodium. 

 Does the endoplasm extend into the pseudopodium? Can you 

 explain how the movement is caused ? 



3. Find a clear space which appears and disappears at inter- 

 vals; this is the contractile vacuole. Determine the length of 

 time between successive contractions. Are the intervals regu- 

 lar? When the vacuole contracts what becomes of the con- 

 tents? What is its function? 



4. Note the oval or rounded nucleus moving with the flowing 

 endoplasm. What is its structure? 



