4 HE AGE NTS. 



Chloroform. 



Chloroform is chiefly used as a solvent for fats, resins, and 

 oils ; also to some extent in the preparation of chloroform balsam. 

 According to VanWisselingh it is a solvent for the various suberin 

 constituents. 



Calcic Chloride. 



This salt in an aqueous solution is used as a mounting fluid 

 and not infrequently it is found useful for clearing. The tissue to 

 be cleared is moistened with a little water and some of the pow- 

 dered salt is then sprinkled on it. It is heated gently until most of 

 the water has been evaporated. The whole is again moistened and 

 mounted in glycerin when it becomes clear. 



Cupric Sulphate. 



The pure salt in an aqueous solution is largely used in the 

 detection of sugars. The test employed by Trommer is as follows : 

 The tissue under examination is allowed to remain in a concen- 

 trated solution of the salt for about ten minutes when it is rinsed 

 with distilled water and placed in a boiling mixture of water and 

 and potassic hydrate. The reaction with cane sugar in the section 

 is to turn the cells containing it a light blue, white with grape 

 sugar (glucose), the reaction causes the cells to become clouded by 

 the deposition of a fine flocculent or granulated orange precipitate 

 of reduced oxide of copper. Dextrine when not mixed with protein 

 compounds assumes a vermilion color. Protein compounds in 

 young cells, with the above tests, turn a violet color. We are thus 

 enabled to detect the presence of two and often three kinds of 

 sugars in this reaction. 



Carbon Disulphide. 



This agent is used chiefly as a solvent for fats, oils, wax, etc., 

 also for carotin, a coloring matter with the same composition as 

 xanthophyll, chlorophyll yellow, etc. 



Carbolic Acid (Phenol). 



This acid is sometimes used as a solvent for fat and fatty oils. 

 When mixed with three parts of turpentine, it makes a good clear- 



