CONTENTS. 



arm 



Microtomes 53 



Reagents 54 



Slides, Covers, Brushes, Paper, Dissect- 

 ing Needles, Razor, etc v54-55 



CARE OF APPARATUS 56 



Cleaning' Instrument, Lenses, etc. 56 



Testing Tissue with Acids 56 



Oiling, Changing Objectives and Ocu- 

 lar 56 



Care of Lenses, Focussing 57 



CARE OF EYES 58 



Bye Shade 59 



Sources of Light 59 



Artitii-ial Light - 59 



MANIPULATION OF APPARATUS 60 



Interpreting Appearances 60 



Cloudiness on the Lenses 60 



Focussing 61 



Optical Sections 61 



Air Bubbles 62 



Oil Globules 62 



MAGNIFICATION 63 



Determination by use of a Camera Lu- 



cida 63 



Determination of Ocular Micrometer 



Ratio 64 



Micron 64 



Tube Length 65 



PRACTICAL EXERCISES 



65 



PART SECOND. 



Laboratory Directions. 



Divisions of the Subject. 



A. Living Cells, (with Protoplasm and Chlorophyll.) 66 



B. Contents of Cells, (the secondary products.) 66 



C. Elementary Tissues 66 



D. The Primary Meristem 66 



E. The Systems of Tissues 66 



F. The Thickening of Stems, etc., (secondary growth) 66 



A. Study of Living Cells 67 



1. THOSE LIVING SEPARATE FROM ONE ANOTHER 67 



a. Protococcus viridia 67 



b. Mother-cells of Pollen, Begonia 68 



c. Mature Pollen Grains, .Malvaceae 70 



d. Culture of Pollen Grains, Tradescantia 71 



2. CELLS IN COLONIES, JOINED TEMPORARILY 72 



a. Spirogyra, (showing Protoplasm, Chlorophyll and Progressive Cell Di- 

 vision) 72 



3. CELLS PERMANENTLY JOINED 74 



A. NOT FORMING TISSUE 74 



Stamen Hairs of Tradescantia 74 



B. FORMING TISSUE 74 



Illustrated by the Majority of the Subsequent Studies 74 



