Xll 



CONTENTS. 



Chlor-iodide of Zinc 11 



Test for Cellulose 11 



Test for Tannin 11 



Action on Fungus Cellulose 11 



HARDENING AGENTS 12 



Alcohol 12 



Dehydrating apparatus 13 



Picric Acid 15 



Chromic Acid 15 



Osmic Acid 15 



Hardening Fluid - 15 



CUTTING AND MOUNTING TISSUES 10 



Free-Hand Sectioning 16 



Softening Hard Tissues 16 



Use of Pith or Cork 16 



Use of Parafflne 17 



Parafflne Method 17 



Construction of Paper Boat 18 



Microtomes 19 



Fixing Sections to Slide 19 



Staining, Mounting, etc 20 



Collodion Method 21 



Hardening, Sectioning, etc 22 



Ether Vapor Bottle 23 



Treatment of Delicate Tissues 24 



STAINING AGENTS 26 



Ammonium Carmine 2<> 



Alum Carmine 26 



Eosiu 27 



Haematoxylin 27 



ANILINE COLORS 27 



Methyl Violet 27 



Methyl Green 27 



Aniline Blue 28 



Magenta 28 



PICRIC ACID 28 



SILVER NITRATK 28 



CLEARING AGENTS 29 



Cedar Oil 20 



Clove Oil 29 



Solvent for Collodion 29 



Use in the Minute Dissections 29 



Origanum Oil 30 



Sandal wood Oil 30 



Carbolic Acid and Turpentine 30 



MOUNTING MEDIA 31 



Aluminum Acetate 31 



Mounting Algae .'51 



Biilsam 31 



Clearing :il 



Preparation of Balsam .. 31 



Treatment for Air Bubbles 31 



Sealing Mounts 32 



Balsam Bottle ... 32 



Carbolic Acid 32 



Calcic Chloride 3~: 



Glycerin Jelly 32 



K Miser's Formula 32 



Glycerin 32 



Use for Fresh Tissues 33 



King's Mounting Medium 33 



Water :: i 



Action on Tissues 33 



CEMENTS 34 



Gold Size 34 



Shellac ' 34 



Ball Cement 34 



Asphalt Varnish 34 



White Zinc Cement 34 



SERIAL SECTIONING 35 



Paralliino, and Collodion Sections 35 



Arrangement on Slide 35 



DOUBLE STAINING 37 



Combination of Stains 37 



Use of Mordant 37 



FLUID MOUNTS 38 



Mount ing in Cells 38 



Construction of Cells 38 



Mounting Fluid 38 



Sealing Mounts 3!i 



Dry Mounts 39 



EQUIPPING OF LABORATORY 40 



Case for Reagents 4O 



Supplies and Their Location 40 



Waste Vessels 4O 



Clearer Bottle 41 



Collection and Preservation of Mate 



rial 42 



Treatment of Soft Tissues 42 



Preservation in Alcohol 42 



Preservation in Collodion :! 



Preservation on Corks or Blocks 43 



THE MICROSCOPE 44 



Description of Instrument 45 Hi 



METHODS OF STUDY 4 7 



Care in Observation 47 



Directions for Drawing 47-48 



I'se of Camera Lucida. 48 



Drawing Material 49 



Page of Drawing Book 51) 



Preservation of Slides 50 



Mailing Boxes 50 



Construct ion of Cabinet 51 



Catalogue of Preparations with Sample 



Card 52 



APPARATUS NEEDED 53 



Microscopes 53 



