74 THE STUDY OF LIVING CELLS. 



Describe the plant and all the phenomena observed, carefully 

 and concisely, including the effects of the staining agents. 



Sachs' Botany, p. 16; Vines Text Book, p. 118; Parker's 

 Biology, p. 192; Goebel, p. 49; Strasburger, p. 246; McAlpine's 

 Charts, pi. iv; 19th Smithsonian Contributions, pi. 14 and 15. 



For method of making permanent mounts of Spirogyra see p. 38. 



CASE III. 



This includes by far the largest number of plants existing. 

 They may be considered under two heads. 



A. THOSE WHICH DO NOT FORM TISSUE, of which, Chara and 

 Nitella may be taken as a type. The same condition exists in 

 stamen hairs, many trichomes, etc. 



B. THOSE WHICH FORM TISSUE, as the higher Cryptoyamia and 

 the Flowering Plants, which will furnish the illustrations under 

 the subsequent heading of T'issues. 



CASE III. (A.) 



ILLUSTRATION FIRST: STAMEN HAIRS of Tradescantia showing 

 PROTOPLASM in MOTION. (Streaming movement.) (Fig. 10.) 



PREPARATION. Remove from a perfectly fresh, newly opened 

 flower a stamen with the attached hairs or trichomes. Place them 

 in water taking particular care not to break or injure the trichomes. 



OBSERVE: 1. The number and/b?v/# of the trichome cells. 



2. The faint pink color of the cell sap, due not to colored 

 granules, but to a red pigment held in solution. 



3. The nucleus. 



4. Slender streams of moving protoplasm. Trace their course. 



5. Estimate their rate of movement. 



For figures of the above see Strasburger, p. 29: Sedgwick and 

 Wilson's Biology, p. 30; Bessey's, p. 12. 



The movement of protoplasm can likewise be studied in the 

 young plant hairs of Cucurbitaceae, in Nitella, or in Vallisneria. 

 Any of the specimens can be mounted in water and treated the 

 same as Tradescantia. 



