58 THE ORIGIN OF LIFE 



first after the process of heating, and if after an 

 interval they are there in abundance and are in- 

 variably stationary, clearly they must have de- 

 veloped in the sites where they are found." 1 



The growth and multiplication of the organisms, 

 however, has often been followed in one or other 

 of two ways. 



(a) In some cases where it has been desired to 

 preserve for a time a particular sample taken from 

 a tube and found to contain organisms, the cover- 

 glass has been at once surrounded with paraffin 

 melting at about 105 F., in order to prevent 

 evaporation of the fluid. Often in such cases, in 

 the course of four or five days it has been found 

 that a distinct multiplication of Torulse or of Bac- 

 teria has taken place. Examples of this are shown 

 in Plate 4, Fig. 23, and Plate 5, Fig. 27. Great 

 multiplication of Torulae had occurred, though the 

 samples had been taken from tubes originally 

 heated to 130 and 135 C. respectively. 



(fo) At other times, especially when doubts have 

 been entertained as to whether what have been 

 seen in the samples have been really Bacilli or 

 Micrococci (Torulse being, of course, always un- 

 mistakable), an inoculation of another sample from 



1 Med. Chir. Trans., vol. xc., 1907, p. 519. 



