CHAPTER III 



NEW EXPERIMENTS INITIATED IN 1909 



As I had seen no account of attempts by others 

 to repeat these experiments, and as I was anx- 

 ious to discover the best proportions in which to 

 use the different ingredients of the two solutions 

 one of which, for convenience of reference, I 

 shall speak of as yellow * and the other as colour- 

 less I made last year, between June and August, 

 105 other experiments with hermetically-sealed 

 vessels heated from 100 to 135 C., in addition 

 to very many other comparative and tentative 

 trials in which the fluids were heated to 100 C. 

 for ten minutes, and were not contained in her- 

 metically-sealed vessels. 2 



In many of these tentative experiments I in- 

 oculated solutions, having the ingredients in dif- 

 ferent proportions, with Bacteria which had been 



1 Though they are often after the heating process rather 

 more red than yellow. 



2 Concerning such experiments see p. 44, note. 



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