60 THE ORIGIN OF LIFE 



the fluid often does not become turbid, but a sedi- 

 ment may distinctly increase in the course of from 

 four to eight days. When a portion of this is 

 removed by a pipette, it will be found to be 

 composed of aggregates of Bacilli, or Bacilli and 

 Cocci, mixed with Torulae, such as are shown in 

 Plates 1-5, Figs. 6, 7, 18, 24, 28. At other times, 

 when there have been no Bacteria, only Moulds 

 may be seen slowly developing at the bottom of 

 the flask, and after a time rising to the surface 

 and developing into distinct specimens of Penicil- 

 lium with crowds of acrospores, as in Plates 3, 

 4, Figs. 19, 22; while, occasionally, where the 

 organisms have been scarce, no result has followed 

 from such inoculations. 



I have several times divided a recently inoculated 

 test fluid into two portions, and have heated one 

 of the portions to 100 C. for one minute, leaving 

 the other half unheated, and have subsequently 

 placed them side by side in the warm chamber. 

 This I have done with samples taken from tubes 

 that had been heated to 130 and also to 135 C., 

 with the result that the fluid in the unheated test 

 flask has speedily become turbid, or shown an in- 

 creasing deposit, while the fluid in the other has 

 alwavs remained clear and revealed no trace of 



