82 ORIGIN OF LOWEST ORGANISMS. 



After forty-eight hours the colour of the infusion was 

 still lighter; there was more turbidity, and some sedi- 

 ment. On microscopical examination, the fluid was 

 found to contain an abundance of Bacteria, Vibrioues 

 and short Leptothrix filaments. 



No. XLI, Hay Infusion + V of Carbolic Acid 



showed no apparent change* after forty-eight hours, 

 and when examined microscopically it revealed no trace 

 of Bacteria, or other organisms. The neck of the flask 

 was then again closed. On the twelfth day the fluid 

 had still undergone no change in appearance, and when 

 examined microscopically, it still showed no trace of 

 organisms, though the fluid was as it had been at the 

 time of the first examination full of minute, undis- 

 solved particles of carbolic acid. 



Fluids loiled for five minutes, and half-filling hermetically 

 Sealed Flasks containing Ordinary Air. 



No. XLIL Hay Infusion, after forty-eight hours, showed 

 no change, and continued to remain quite clear and 

 free from deposit until the twelfth day, when it was 

 examined microscopically. No organisms of any kind 

 could be detected. 



No. XLIII. Hay Infusion -)- gV part of Carbolic 



Acid showed no apparent change t for the first five 

 days, though, on the sixth day, a slight deposit was 

 noticed at the bottom of the flask. The deposit had 

 increased, and was well-marked by the twelfth day, 



* The fluid had been rendered paler and turbid from the first, 

 by the addition of the carbolic acid. 



f The alteration in colour was less marked than in the similar 

 mixture which had not been boiled, though the turbidity was 

 just as obvious. 



