ORIGIN OF LO WES T OR GA NISMS. 7 1 



e. Fluid (in vacuo) in a Flask, the Neck of which was her- 

 metically Sealed by means of the Blowpipe Flame during 

 Ebullition. 



No. XIII. Urine in forty-four hours showed a very 

 slight amount of sediment. During the next two days 

 the sediment very slightly increased, but was still small 

 in amount. At the expiration of fifteen days, no further 

 increase in the turbidity having taken place, the fluid 

 was examined. The vacuum was still partially pre- 

 served, as evidenced by the rapid inbending of a portion 

 of the neck of the flask after it had been carefully made 

 red-hot. When opened, the odour of the fluid was 

 stale, but not foetid, and its reaction was still faintly 

 acid. On microscopical examination Bacteria and 

 Toruld were found in tolerable abundance. 



No. XIV.- Hay Infusion in forty-four hours showed a 

 very slight amount of turbidity. In sixty-eight hours 

 the turbidity was most marked, and there was also a 

 small amount of sediment. In another twenty-four 

 hours it was noticed that the colour of the fluid had 

 become much lighter, whilst the turbidity and sediment 

 had increased. It subsequently continued in much the 

 same state, and the flask was opened on the sixteenth 

 day. The vacuum was found to be almost wholly 

 impaired, whilst the odour of the fluid was sour, and 

 not at all hay-like. On microscopical examination 

 Bacteria, Vibriones, Leptothrix, and Tornla, were found 

 in abundance, and the former were very active. 



No. XV. Turnip Infusion after forty-eight hours showed 

 a well-marked turbidity. In seventy-two hours the tur- 

 bidity was more marked, and there was a slight amount 

 of sediment. The turbidity also increased during the 



