72 ORIGIN OF LO WEST ORGA NISMS. 



next twenty-four hours ; though, after that, the infusion 

 seemed to undergo no further change. The flask 

 remained in the warm bath for fifteen days, when the 

 fluid was examined. Its odour was not foetid, but was 

 somewhat like that of baked turnip. Bacteria and 

 Vibriones existed in abundance, though their movements 

 were extremely languid. 



SECOND SET OF EXPERIMENTS (XVI.-XXI.). 



b. Fluid in contact with Ordinary Air and its Particles ; Neck 

 of Flask Sealed after the Fluid had become Cold. 



No. XVI. Simple Turnip Infusion in twenty-four 

 hours had undergone no apparent change. In thirty- 

 six hours there was slight turbidity, and in forty-eight 

 hours this was most marked and uniform. When the 

 flask was opened, after seventy-two hours, there was an 

 outrush of very foetid gas ; the reaction of the fluid was 

 acid, and, when examined microscopically, it was found 

 to contain multitudes of very languid Bacteria. 



No. XVII. Neutralized Infusion of Turnip -f- i gr. 

 of Cheese,* in thirty-six hours showed a well-marked 

 pellicle.t When the flask was opened, after seventy-two 

 hours, there was a violent outrush of gas, though the 

 fluid was still neutral. Portions of the thick pellicle 

 were found, on microscopical examination, to be made 

 up of Bacteria, Vibriones, and an abundance of long, 



* The filtered infusion of turnip was neutralized by liquor 

 potassse. The cheese (Cheddar) was new and not in the least 

 mouldy. 



f The fluid itself being somewhat opaque, the first stages of 

 increased turbidity from presence of Bacteria could not be 

 detected. 



