ORIGIN OF LO WEST OR CAN ISMS. 9 1 



microscopical examination were found to be aggrega- 

 tions of Bacteria. 



Ammoniacal Solutions boiled (at 212 F.\ and exposed to 

 Air in Flasks whose Open Necks were only loosely 

 covered with Paper Caps : subsequent Inoculation. 

 (Temp. 75 -8 5 F.\ 



No. LIX. Ammonic Tartrate Solution. The fluid 

 remained quite clear, and free from all trace of tur- 

 bidity up to the ninth day, when it was inoculated with 

 some living Bacteria. In fifty hours after the inocu- 

 lation there was a very faint opalescence of the fluid, 

 which, in another 24 hours, had become much more 

 marked. On microscopical examination it was found 

 to contain multitudes of Bacteria. 



No. LX. Ammonic Tartrate and Sodic Phosphate 

 Solution. After four days the fluid was still quite 

 clear. In seven days no trace of general turbidity, 

 though there was a minute dirty-grey aggregation 

 about -jL" in diameter at the bottom of the flask. On 

 the sixteenth day the grey aggregation had very slightly 

 increased in size, though the fluid above was still per- 

 fectly clear. The grey mass was removed by a small 

 pipette, and, on microscopical examination, it was found 

 to be composed of an aggregation of minute extraneous 

 fibres, mixed with blackish particles and amorphous 

 granular matter, in which were growing many Torula- 

 cells in all stages of development, and also a minute 

 mycelium composed of branched Leptothrix3fc& fibres.* 



A deposit of this kind is almost invariably found in such 

 solutions after their fermentability has been lowered by previous 

 boiling. Growth takes place very slowly in these cases, and also 

 when similar boiled fluids are contained /;/ vacuo. 



