3] 



ACCLIMATIZATION 



87 



Athough there has been a gradual improvement in methods, 

 the conditions other than that of concentration have too often 

 been omitted from consideration. The omission of the tempera- 

 ture of the experiment solutions is especially unfortunate, for 

 according to GOGORZA ('91, p. 270), acclimatization is more 

 easily effected at a low temperature than at a high one. 



Of the papers mentioned above, that of MASSART is especially 

 worthy of extended notice from its quantitative nature. He 

 subjected cysts of Ciliata to various concentrations of KNO 3 

 and noted the effect upon the protoplasm. In the following 

 tables, the first line of numbers names the solution in parts of 

 the molecular weight expressed in grammes. The symbols in 

 the columns headed by these numbers have the following signifi- 

 cations: 0, no effect; v, the cysts possess a large vacuole whose 

 pulsations are infrequent; v p, the vacuole is still prominent 

 but plasmolysis is occurring ; p, the plasmolysis is more marked 

 and the vacuole is gone; P, the plasmolysis is so marked that 

 the form of the infusorian is lost. The results given in the 

 third and fourth lines were obtained from individuals acclimated 

 for 22 hours to a 1.8 MW % and to a 3 MW % solution of 

 KNO 3 respectively. The observations were made immediately 

 after immersion of the cysts. No mention is made of the 

 temperature. 



TABLE X VORTICELLA 



TABLE XI COLPODA 



